Advance your proteomic and metabolomics workflows using fluorous tagging and separation to enrich biological samples in specific substrates or molecular classes. The tagging process labels the target molecules with a perfluorocarbon domain within the mixture using standard chemistries (e.g., an NHS ester to tag amino residues). The fluorous tagged molecules can then be selectively retained on FluoroFlash silica gel while non-tagged species are washed away. Elution of the fluorous silica gel to release the fluorous tagged substrates then provides the enriched sample. The process is similar in concept to affinity separations utilizing biotin or His tags, providing samples of dramatically reduced complexity which can then be analyzed using either MS or gel based strategies.
Fluorous tagged molecules can also be immobolized on fluorous modified surfaces, such as glass, for microarray formation. Qualitative and quantitative binding assays along with enzyme activity assays have all been demonstrated using fluorous immobilized substrates.
To learn more about fluorous techniques in life sciences applications, please click on the application of interest.