Fluorous solid phase extraction (F-SPE) is used for quick separations of reaction mixtures involving fluorous catalysts, reagents, protecting groups, tags, and scavengers (1-6). F-SPE is used for quick separations of reaction mixtures involving FluoroFlash® tags, reagents, scavengers and catalysts. The separation of fluorous compounds by F-SPE is a reliable and generic procedure that more greatly resembles filtration than chromatatography. The facile separation depends primarily on the presence or absence of a light fluorous tag, not polatity or other molecular features that control traditional chromatography.
A simple two-step separation of fluorous compounds involves the loading of a reaction mixture containing the fluorous-tagged molecule on to an F-SPE cartridge, washing the non-fluorous compounds away using a fluorophobic wash, then washing the fluorous compounds with a fluorophilic wash. Please consult the F-SPE application note for a more detailed procedure.
FluoroFlash® SPE cartridges are pre-packed in a variety of formats with a proprietary fluorous silica gel. A Fluorous Dye Separation Kit (cited on the F-SPE Application Note) is available to demonstrate how the F-SPE works. If you require a particular format, i.e. specific cartridge size, multi-well plate, etc., please contact FTI.
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2. Curran, D. P. Handbook of Fluorous Chemistry, 2004, 101-155.
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6. Curran, D. P.; Hadida, S.; He, M. J. Org. Chem. 1997, 62, 6714-6715.