Fluorous Applications

 

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Fluorous Microarrays

Benefits of Fluorous Microarrays

  • Create custom microarrays with a wide variety of molecules
  • Increase signal-to-noise ratio
  • Simplify your workflow through reduction of washing and blocking steps
  • Control the specific display orientation to reduce variability
  • Obtain low and uniform background fluorescence
  • Achieve low non-specific binding allowing the use of cell lysates or purified proteins

Fluorous based microarrays provide a unique surface chemistry which allows you to obtain superior results while simplifying workflows. Fluorous immobilization is a non-covalent technique which has many of the benefits of covalent immobilization, but eliminates many of the washing and blocking steps required. Simply spot, dry, and incubate. The resultant arrays possess high signal-to-noise, low and uniform background fluorescence, and low non-specific binding. To date small molecules, peptides, and carbohydrate arrays have all been formed using fluorous immobilization [1-3]. The low non-specific binding of the fluorous surface allows the microarrays to be interrogated with cell lysates in addition to purified proteins and enzymes. Fluorous microarray data has been validated against other methods including biochemical assays and surface plasmon resonance(SPR)[4]. Binding assays, both qualitative and quantitative, along with enzyme activity assays[5] have all been conducted using fluorous microarrays.

Microarray

Fluorous tagged content can be generated from either direct synthesis or modification of existing content. Fluorous chemistry is the only strategy which allows synthesis, purification, and immobilization using a single tag in a streamlined process with no other chemical manipulations. Go directly from chemical reactions to purification to microarray formation, linking high quality content generation with high quality assay results.

Start Building Your Custom Microarrays

Fluorous Technologies Inc. offers all the tags required for content generation along with fluorous modified glass slides for microarray formation. Our slides are available in either plain or bar-coded versions are made of the highest quality microarray glass. Please view our product line using the links in the Related Products section to the left. Read more details about fluorous microarrays in the documents and pages listed under For More Information.

Selected References

  • Ko, K.S.; Jaipuri, F.A.; Pohl, N.A. “Fluorous-Based Carbohydrate Microarrays” J. Am. Chem. Soc. 2005, 127, 13162-13163.
  • Jaipuri, F.A.; Collet, B.Y.M.; Pohl, N.L., “Synthesis and Quantitative Evaluation of Glycero-D-manno-Heptose Binding to Concanavalin A by Fluorous-Tag Assistance” Angewandte Chemie International Edition 2008, DOI: 10.1002/anie.200704262.
  • Nicholson, R.L.; Ladlow, M.L.; Spring, D. "Fluorous tagged small molecule microarrays" Chem. Commun., 2007, 38, 3906.
  • Vegas, A.J.; Bradner, J.E.; Tang, W.; McPherson, O.M.; Greenberg, E.F.; Koehler, A.N.; Schreiber, S.L. “Fluorous-Based Small-Molecule Microarrays for the Discovery of Histone Deacetylase Inhibitors” Angewandte Chemie International Edition 2007, 46, 42, 7960.
  • Northen, T. R.; Lee, J.-C.; Hoang, L.; Raymond, J.; Hwang, D.-R.; Yannone, S. M.; Wong, C.-H.; Siuzdak, G., Proc. Nat. Acad. Sci. 2008, 105, (10), 3678-3683.